Aim. The study was conducted to investigate the antibacterial and antiasthmatic effects of Nandina domestica leaf extract, to find\nout its active components, and to assess its safety issue. Methods. (1) Solid-phase agar dilution method was used for antibacterial\nactivity test of nandina leaf extract and the change of bacterial morphology after treatment was observed under the transmission\nmicroscope; (2) guinea pig model of asthma was used to test the asthma prevention effect of nandina leaf extract; (3) alkaloids and\nflavoneswere separated fromnandina leaf extract andwere further analyzed with HPLC-MS; (4) micemodel was used to assessment\nof the safety issue of nandina leaf extract. Results. (1) Nandina leaf extract inhibited the growth of bacteria and destroyed bacterial\nmembrane; (2) nandina leaf extract alleviated animal allergy and asthma; (3) the components reextracted by ethyl acetate were\nactive, in which alkaloids inhibited Gram-positive bacteria and prevented asthma and flavones inhibited Gram-negative bacteria;\n(4) nandina leaf extract had no toxic effect on mice. Conclusion.Nandina leaves inhibit bacterial growth and prevent asthma through\nalkaloids and flavones, which had integrated function against chronic bronchitis. This study provided theoretical basement for\nproducing new Chinese medicine against chronic bronchitis.
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